Restriction Enzymes
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Filtered Search Results
Thermo Scientific™ FastDigest AatII
Cut at GACGT^C sites with Thermo Scientific™ FastDigest™ AatII, which performs best at 37°C in 5-15 minutes using universal FastDigest™ Buffer (Isoschizomers: ZraI).
Thermo Scientific™ FastDigest SgsI
Cut at GG^CGCGCC sites with Thermo Scientific™ FastDigest™ AscI (SgsI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PalAI).
Thermo Scientific™ FastDigest SfiI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest KspAI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest AdeI
Cut at CACNNN^GTG sites with Thermo Scientific™ FastDigest™ DraIII (AdeI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ BclI (10 U/μL)
Cut at T^GATCA sites with BclI restriction enzyme, which performs best at 55°C in G buffer (Isoschizomers: FbaI, Ksp22I).
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ NheI (10 U/μL)
The NheI restriction enzyme recognizes G^CTAGC sites and cuts best at 37°C in Tango buffer (Isoschizomers: AsuNHI, BmtI).
| Concentration | 10 U/μL |
|---|
Thermo Scientific™ XmaJI (AvrII) (10 U/μL)
Cut at C^CTAGG sites with XmaJI (AvrII) restriction enzyme, which functions best at 37°C in Tango buffer (Isoschizomers: AspA2I, AvrII, BlnI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | XmaJI (AvrII) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ FspAI (5 U/μL)
The FspAI restriction enzyme recognizes RTGC^GCAY sites and cuts best at 37°C in O buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | FspAI |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer O |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 5 U/μL |
Thermo Scientific™ SspI (10 U/μL)
The SspI restriction enzyme recognizes AAT^ATT sites and cuts best at 37°C in G buffer.
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | Ssp I |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer G |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ Eco72I (PmlI) (10 U/μL)
Cut at CAC^GTG sites with Eco72I (PmlI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AcvI, BbrPI, PmaCI, PmlI, PspCI).
Thermo Scientific™ MauBI (5 U/μL)
Cut T CG^CGCGCG sites with MauBI restriction enzyme, which performs best at 37°C in Tango buffer.
Thermo Scientific™ SsiI (AciI) (10 U/μL)
Cut at CCGC(-3/-1)^ sites with SsiI (AciI) restriction enzyme, which functions best at 37°C in O buffer (Isoschizomers: AciI, BspACI).
Thermo Scientific™ AjuI (5 U/μL)
Cut at ^(7/12)GAA(N)7TTGG(11/6)^ sites with AjuI restriction enzyme, which performs best at 37°C in R (+SAM) buffer.
Thermo Scientific™ HpyF3I (DdeI) (10 U/μL)
The HpyF3I (DdeI) restriction enzyme recognizes C^TNAG sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstDEI, DdeI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | HpyF3I (DdeI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |