Restriction Enzymes
Thermo Scientific™ Mva1269I (BsmI) (10 U/μL)
The Mva1269I (BsmI) restriction enzyme recognizes GAATGC(1/-1)^ sites and cuts best at 37°C in R buffer (Isoschizomers: BsaMI, BsmI, PctI).
Thermo Scientific™ Bsp68I (NruI) (10 U/μL)
Cut at TCG^CGA sites with Bsp68I (NruI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: BtuMI, NruI).
Thermo Scientific™ FastDigest SgsI
Cut at GG^CGCGCC sites with Thermo Scientific™ FastDigest™ AscI (SgsI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PalAI).
Thermo Scientific™ MnlI (10 U/μL)
The MnlI restriction enzyme recognizes CCTC(7/6)^ sites and cuts best at 37°C in G buffer.
Thermo Scientific™ FastDigest SfaAI
Cut at GCGAT^CGC sites with Thermo Scientific™ FastDigest™ AsiSI (SfaAI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ EcoRI, HC (50 U/μL)
The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.
Thermo Scientific™ BseMI (BsrDI) (5 U/μL)
Cut at GCAATG(2/0)^ sites with BseMI (BsrDI) restriction enzyme, which performs best at 55°C in R buffer (Isoschizomers: Bse3DI, BsrDI).
Thermo Scientific™ FastDigest XmiI
Cut at GT^MKAC sites with Thermo Scientific™ FastDigest™ AccI (XmiI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FblI).
Thermo Scientific™ SsiI (AciI) (10 U/μL)
Cut at CCGC(-3/-1)^ sites with SsiI (AciI) restriction enzyme, which functions best at 37°C in O buffer (Isoschizomers: AciI, BspACI).
Thermo Scientific™ Eco31I (BsaI) (10 U/μL)
The Eco31I (BsaI) restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in G buffer (Isoschizomers: BsaI, Bso31I, BspTNI).
Thermo Scientific™ AjuI (5 U/μL)
Cut at ^(7/12)GAA(N)7TTGG(11/6)^ sites with AjuI restriction enzyme, which performs best at 37°C in R (+SAM) buffer.
Thermo Scientific™ SdaI (SbfI) (10 U/μL)
The SdaI (SbfI) restriction enzyme recognizes CCTGCA^GG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: SbfI, Sse8387I).
Thermo Scientific™ PscI (PciI) (10 U/μL)
The PscI (PciI) restriction enzyme recognizes A^CATGT sites and cuts best at 37°C in Tango buffer (Isoschizomers: PciI).
Thermo Scientific™ Eco47III (AfeI) (10 U/μL)
The Eco47III (AfeI) restriction enzyme recognizes AGC^GCT sites and cuts best at 37°C in O buffer (Isoschizomers: AfeI, Aor51HI).
Thermo Scientific™ FastDigest BseJI
Cut at GATNN^NNATC sites with Thermo Scientific™ FastDigest™ BsaBI (BseJI), which performs best using universal FastDigest Buffer (Isoschizomers: Bse8I).